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WSE-6370/6370K LuminoGraph III Lite_Chemiluminescence Imaging System

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WSE-6370/6370K LuminoGraph III Lite_Chemiluminescence Imaging System

WSE-6370/6370K LuminoGraph III Lite is a Chemiluminescence Imaging System with the Purpose and Application: 1) Western blot, Dot blot 2) DNA gel 3) Bioluminescence 4) Protein gel (Colorimetric)

Product model:

WSE-6370/6370K

Product description

Parameters

WSE-6370/6370K LuminoGraph III Lite

_Chemiluminescence Imaging System

Purpose and Application

Western blot, Dot blot
DNA gel
Bioluminescence
Protein gel (Colorimetric)

Features

AutoExposure
→ The suitable exposure time is automatically calculated and images are photographed and saved
Auto contrast・Inverse image
→Control software and image analysis software is available for auto contrast and inverse image functions
Simple icon, User-friendly
Auto exposing, Sum, etc. Various detection mode
User registration and personal folder: Simple an image file management
6 mega pixels high sensitive cooled CCD camera enables high resolution and sensitivity
F 0.8 high sensitive lens
CS Analyzer -ATTO Original analysis software
Windows PC installation
Housekeeping protein ratio Normalization function
Free updating(Software) service
Image analysis function
Revision of distortion bands pattern
Quantitative analysis of band signal intensities

LuminoGraph III Lite system

Easy to Use

User registration and file management

Register user ID and manage imaging files individually. Imaging files are saved in the logged-in user’s folder.

The captured image displayed in chronological order. You can export or delete multiple images by select a date.

Intuitive button

Select Application

Easy to capture. The menu is configured according to the applications

Enable Auto Exposure

The Auto Exposure function can be applied to all applications.

Easy to set for Fluorescent dye

One-click optimal settings for fluorescent dye

Simple Contrast adjustment

Powerful Auto Exposure

Auto Merge

Western blot auto exposure provides Auto Merge function. Easily check the M.W. of the target protein.

Easily capture low-sensitivity images with Set Area function

Many Imaging systems that support Auto Exposure capture images basis on the strongest signal to avoid saturation. For this reason, it may be difficult to detect target protein if you capture an image with a big difference in sensitivity or the nonspecific band's signal is strong.

ATTO Imaging systems, including the LuminoGraph III Lite, have Set Area function. Bands that exist in a selected area are detected. Images are saved as many as area selected.

Capture an image of samples at once with large differences in expression level

When capturing multiple blots, the exposure time is calculated for each specified area and each images are taken. Even the difference in the expression level is large, good results are obtained.

Detect samples with weaker sensitivity

If the target protein’s signal is weaker than other proteins such as non-specific bands, it’s difficult to detect. In this case, you can detect the target protein by setting the area.

High Sensitive Imaging

Improved performance

Performance is higher than our previous models. Noise is greatly reduced and sensitivity is increased. Even if you increase the binning level to detect weak signals, the device also detects the band cleanly.

High sensitivity

Adopted the lens and sensor that are the same as our high-end model. Lightweight, cost-effective, and high performance

F 0.8 lens suitable for high sensitivity imaging

Theoretically, the light collection efficiency increases as the inverse of the square of the F. But, the smaller the F value, the greater the gap between the theoretical value and measured value. This is due to the effect of microlens on the surface of the sensor. The LuminoGraph III Lite features a high sensitivity F0.8 fixed focal lens, with little distortion and maximum brightness.

Highly Reliable Results

Powerful quantification and wide dynamic range

A signal detection proportional to the amount of protein is required for the quantification of Western blot, and a wide dynamic range is required to detect weak signals.

The right graph shows the signal measured using a reference light source emitting a faint light. You can see the linearity of R2 value over 0.999.

The Luminograph III Lite has excellent lineality for the wide dynamic range, so it can be quantified simultaneously even if the difference in the expression level is large.

Superior reproducibility

To trust the results of the images captured, reproducibility is required that detect the same amount of signals equally.

The table on the left is the uncertainty produced by repeatedly captured images using a reference light source (WSL-1200 Kohshifundam) and analyzing the intensity of the image. It has excellent reproducibility within ± 0.2% uncertainty

WSL-1200 KohshiFundam

WSL-1200 KohshiFundam, our reference light source for validation, was used for reliability test.

1) Standard light source for verification with specified luminous flux

2) 11 step variation in light volume from 1/1 to 1/1024 by PWM (Pulse Width Modulation) control

3) Deal with the linearity test of photometric equipment

4) Suitable for reproducibility testing, as the amount of light is equal even with changes in environmental temperature

Quantitative Analysis

Densitometry CS Analyzer 4

This software performs various analysis such as intensity measurement, M.W. analysis, pI analysis, and relative quantity, etc. by selecting analysis modes according to sample types such as Lane, Spot, and Plate.

It also has the ability to adjust images such as image rotation, contrast adjustment, and merge, and the adjusted images can be stored in various formats.

You can save the analysis results as a CSV file that is available in MS Excel and save data in report format, including images and Standard curve, as PDF files.

Lane Analysis

Quantification of DNA gels or protein gels. Analyze bands and background automatically and calculate the intensity of bands of each lane.

Spot Analysis

Quantification of regions of interesting in western blotting, culture cells, or living organisms.

Plate Analysis

Quantification samples in microplate, array, or dot blotting.

Normalization

We support normalization that compares the relative abundance of a specific protein of blots that are captured separately. Use CS Analyzer instead of inconveniently calculate yourself.

Free Rotation