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EV Isolation Kit(for CCS & Urine)

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Product description

Product    Description

Extracellular vesicles (EVs), a family of membrane enclosed vesicles secreted by various types of cells, are involved  in cell-to-cell communication by transferring cargos, such as proteins, metabolites, and nucleic acids. A key caveat to the clinical exploitation of EVs is that traditional method of choice for EV isolation, the ultracentrifugation, requires  prohibitively long time and efforts, this is even true for large scale studies and less experienced researchers who want to isolate EVs in a timely and cost-effective manner with a required yield and at a suitable scale. Therefore, a methodology that will allow the rapid isolation of EVs with high yield would greatly facilitate such applications.
One familiar method that is increasingly being applied to isolate EVs is the use of precipitants such as polyethylene glycol (PEG) combined with centrifugation to obtain pellet EVs for subsequent processing. Precipitation method has several advantages; (i) familiar to use, (ii) uses common laboratory equipment, (iii) suitable for both small and large sample volumes, (iv)high yield. However, it also has the limitations: (i) polymeric contaminants, (ii) extended processing time, (iii) complicated clean-up steps, (iv) affecting downstream analysis and quantification.
ExoPRISMTM EV Isolation Kit, as a novel precipitation method, is introduced to overcome those hurdles by using low-molecular-weight  electrolytes  instead  of  the  PEG  polymer. (i) The  isolated  and  enriched  EV  sample  by ExoPRISMTM doesn't have polymeric contaminants. (ii) Due to the quick reaction of our reagents and the sample within  10  minutes, the overall  processing time  is significantly  reduced. (iii)  ExoPRISMTM reagents can  be easily removed from the enriched EVs and allows elution by the buffer solution of your choice. (iv) As a result, ExoPRISMTM has no effect on downstream analysis or applications.
The kits include two types of reagents: Reagent B and Reagent C, which are optimized for CCS and urine samples, respectively. The Reagent B is an EV precipitation solution and the Reagent C is a washing solution.

List of Components 

"Reagent B and C should be stored at room temperature. If there are precipitates in Reagent B, please warm it at 37 ℃ in the stirring  incubator  until the  precipitates disappear with the  naked eye, and  cool  it down at  room  temperature before use.



Usage of Reagents

The volume of reagents needed was calculated based on 5 ml of sample. Therefore, additional adjustment of the reagents should be required based on the sample volume.


The reagent volumes are calculated based on 5 mL of CCS or urine. The mixing ratio between sample and Reagent B should be kept at 1:1.Depending on the variety of biological samples, you can adjust the volume of Reagent C.

ExoPRISM  EV  lsolation  Kit  Protocol  





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